biotinylated antimouse rabbit igg (Vector Laboratories)
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Vector Laboratories
biotinylated antimouse rabbit igg
Biotinylated Antimouse Rabbit Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1262 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+antimouse+rabbit+igg/Biotinylated+Horse+Anti-Rabbit+IgG+Antibody/pm41522455-50-117-122
Average 96 stars, based on 1262 article reviews
Biotinylated Antimouse Rabbit Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1262 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+antimouse+rabbit+igg/Biotinylated+Horse+Anti-Rabbit+IgG+Antibody/pm41522455-50-117-122
Average 96 stars, based on 1262 article reviews
biotinylated antimouse rabbit igg - by Bioz Stars,
2026-09
96/100 stars
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In Vitro:Article Title: Combination of Atelocollagen Gel With Induced Pluripotent Stem Cell-Derived Tenocyte for Rotator Cuff Tendon Regeneration in a Rat Model. Article Snippet: After 5 mL of fluorescent antibodies (CD34, CD45, CD73, and CD105; eBioscience) were added and mixed, the cells were rewashed with PBS and incubated in the dark for 45 minutes at 2 to 8 C. Then, 1 3 106 cells were added to 1 mL of PBS containing 1% to 2% FBS, mixed, put into a fluorescenceactivated cell sorter (FACS) tube, and analyzed on a FACS Aria Fusion (BD Bioscience). .. In Vitro Immunofluorescence Staining iPSC-TCs were seeded in a 4-well culture slide (2 3 104 cells per well), cultured for 3 days, fixed with 4% paraformaldehyde for 10 minutes, washed with PBS, and treated with 0.1% Triton X-100 for 8 minutes at 4 C. To prevent nonspecific antigen-antibody binding after the wash with PBS, a reaction with 10% normal horse serum (s-1000, Vector Laboratories, Inc) was conducted for 1 hour, followed by overnight treatment with antibodies for scleraxis (ab58655, 1:100; Abcam), tenomodulin (ab203676, 1:100; Abcam), type I collagen (NB600-450, 1:100; Novus Biologics), vimentin (1:100 Sigma-Aldrich), and octamer-binding transcription factor 4 (OCT4, 1:100; Sigma-Aldrich; Merck Millipore) at 4 C. The cells were treated with a biotin-attached secondary antibody, Immunofluorescence:Article Title: Combination of Atelocollagen Gel With Induced Pluripotent Stem Cell-Derived Tenocyte for Rotator Cuff Tendon Regeneration in a Rat Model. Article Snippet: After 5 mL of fluorescent antibodies (CD34, CD45, CD73, and CD105; eBioscience) were added and mixed, the cells were rewashed with PBS and incubated in the dark for 45 minutes at 2 to 8 C. Then, 1 3 106 cells were added to 1 mL of PBS containing 1% to 2% FBS, mixed, put into a fluorescenceactivated cell sorter (FACS) tube, and analyzed on a FACS Aria Fusion (BD Bioscience). .. In Vitro Immunofluorescence Staining iPSC-TCs were seeded in a 4-well culture slide (2 3 104 cells per well), cultured for 3 days, fixed with 4% paraformaldehyde for 10 minutes, washed with PBS, and treated with 0.1% Triton X-100 for 8 minutes at 4 C. To prevent nonspecific antigen-antibody binding after the wash with PBS, a reaction with 10% normal horse serum (s-1000, Vector Laboratories, Inc) was conducted for 1 hour, followed by overnight treatment with antibodies for scleraxis (ab58655, 1:100; Abcam), tenomodulin (ab203676, 1:100; Abcam), type I collagen (NB600-450, 1:100; Novus Biologics), vimentin (1:100 Sigma-Aldrich), and octamer-binding transcription factor 4 (OCT4, 1:100; Sigma-Aldrich; Merck Millipore) at 4 C. The cells were treated with a biotin-attached secondary antibody, Staining:Article Title: Combination of Atelocollagen Gel With Induced Pluripotent Stem Cell-Derived Tenocyte for Rotator Cuff Tendon Regeneration in a Rat Model. Article Snippet: After 5 mL of fluorescent antibodies (CD34, CD45, CD73, and CD105; eBioscience) were added and mixed, the cells were rewashed with PBS and incubated in the dark for 45 minutes at 2 to 8 C. Then, 1 3 106 cells were added to 1 mL of PBS containing 1% to 2% FBS, mixed, put into a fluorescenceactivated cell sorter (FACS) tube, and analyzed on a FACS Aria Fusion (BD Bioscience). .. In Vitro Immunofluorescence Staining iPSC-TCs were seeded in a 4-well culture slide (2 3 104 cells per well), cultured for 3 days, fixed with 4% paraformaldehyde for 10 minutes, washed with PBS, and treated with 0.1% Triton X-100 for 8 minutes at 4 C. To prevent nonspecific antigen-antibody binding after the wash with PBS, a reaction with 10% normal horse serum (s-1000, Vector Laboratories, Inc) was conducted for 1 hour, followed by overnight treatment with antibodies for scleraxis (ab58655, 1:100; Abcam), tenomodulin (ab203676, 1:100; Abcam), type I collagen (NB600-450, 1:100; Novus Biologics), vimentin (1:100 Sigma-Aldrich), and octamer-binding transcription factor 4 (OCT4, 1:100; Sigma-Aldrich; Merck Millipore) at 4 C. The cells were treated with a biotin-attached secondary antibody, Cell Culture:Article Title: Combination of Atelocollagen Gel With Induced Pluripotent Stem Cell-Derived Tenocyte for Rotator Cuff Tendon Regeneration in a Rat Model. Article Snippet: After 5 mL of fluorescent antibodies (CD34, CD45, CD73, and CD105; eBioscience) were added and mixed, the cells were rewashed with PBS and incubated in the dark for 45 minutes at 2 to 8 C. Then, 1 3 106 cells were added to 1 mL of PBS containing 1% to 2% FBS, mixed, put into a fluorescenceactivated cell sorter (FACS) tube, and analyzed on a FACS Aria Fusion (BD Bioscience). .. In Vitro Immunofluorescence Staining iPSC-TCs were seeded in a 4-well culture slide (2 3 104 cells per well), cultured for 3 days, fixed with 4% paraformaldehyde for 10 minutes, washed with PBS, and treated with 0.1% Triton X-100 for 8 minutes at 4 C. To prevent nonspecific antigen-antibody binding after the wash with PBS, a reaction with 10% normal horse serum (s-1000, Vector Laboratories, Inc) was conducted for 1 hour, followed by overnight treatment with antibodies for scleraxis (ab58655, 1:100; Abcam), tenomodulin (ab203676, 1:100; Abcam), type I collagen (NB600-450, 1:100; Novus Biologics), vimentin (1:100 Sigma-Aldrich), and octamer-binding transcription factor 4 (OCT4, 1:100; Sigma-Aldrich; Merck Millipore) at 4 C. The cells were treated with a biotin-attached secondary antibody, Binding Assay:Article Title: Combination of Atelocollagen Gel With Induced Pluripotent Stem Cell-Derived Tenocyte for Rotator Cuff Tendon Regeneration in a Rat Model. Article Snippet: After 5 mL of fluorescent antibodies (CD34, CD45, CD73, and CD105; eBioscience) were added and mixed, the cells were rewashed with PBS and incubated in the dark for 45 minutes at 2 to 8 C. Then, 1 3 106 cells were added to 1 mL of PBS containing 1% to 2% FBS, mixed, put into a fluorescenceactivated cell sorter (FACS) tube, and analyzed on a FACS Aria Fusion (BD Bioscience). .. In Vitro Immunofluorescence Staining iPSC-TCs were seeded in a 4-well culture slide (2 3 104 cells per well), cultured for 3 days, fixed with 4% paraformaldehyde for 10 minutes, washed with PBS, and treated with 0.1% Triton X-100 for 8 minutes at 4 C. To prevent nonspecific antigen-antibody binding after the wash with PBS, a reaction with 10% normal horse serum (s-1000, Vector Laboratories, Inc) was conducted for 1 hour, followed by overnight treatment with antibodies for scleraxis (ab58655, 1:100; Abcam), tenomodulin (ab203676, 1:100; Abcam), type I collagen (NB600-450, 1:100; Novus Biologics), vimentin (1:100 Sigma-Aldrich), and octamer-binding transcription factor 4 (OCT4, 1:100; Sigma-Aldrich; Merck Millipore) at 4 C. The cells were treated with a biotin-attached secondary antibody, |